发明名称 |
MELTING TEMPERATURE DEPENDENT DNA AMPLIFICATION |
摘要 |
A method for the selective amplification of at least one target nucleic acid in a sample comprising a mixture of at least one target nucleic acid and at least one non-target nucleic acid. The method comprises: a nucleic acid denaturation step, wherein the denaturation step is carried out at a temperature at or above the melting temperature of the at least one target nucleic acid but below the melting temperature of the at least one non-targe t nucleic acid an amplification step using at least one amplification primer.< /SDOAB>
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申请公布号 |
CA2477574(A1) |
申请公布日期 |
2003.09.04 |
申请号 |
CA20032477574 |
申请日期 |
2003.02.26 |
申请人 |
COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION |
发明人 |
CLARK, SUSAN JOY;MOLLOY, PETER LAURENCE;RAND, KEITH |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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