NOVEL TRANS-VIRAL VECTORS COMPRISING MULTICISTRONIC EXPRESSION CONSTRUCTS AND METHODS OF USE

摘要

The present invention provides novel methods and compositions for the expression of a polypeptide of interest. In particular, the methods and compositions of the invention combine a trans-viral vector system (Figure 1) with a gene transfer vector having at least one DNA expression cassette comprising multiple open reading frames separated by internal ribosomal entry sites (IRES) or an active fragment or variant thereof.