发明名称 |
METHOD OF PERFORMING SUBTRACTIVE HYBRIDIZATION |
摘要 |
The subject invention relates to improvements in nucleic acid isolation, and more particularly, relates to modifications to the subtractive hybridization method and to reagents such as oligonucleotides that are useful when performing the method. The present invention provides a modified subtractive hybridization method termed Selectively Primed Adaptive Driver-RDA ("SPAD-RDA"), which utlises a driver-versus-driver subtraction control performed in parallel with the driver-versus-tester subtraction step. The products of the driver control subtraction from each round can be used as the driver of the subsequent round. |
申请公布号 |
WO0210458(A3) |
申请公布日期 |
2003.09.04 |
申请号 |
WO2001US24480 |
申请日期 |
2001.08.02 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
BIRKENMEYER, LARRY, G.;LEARY, THOMAS, P.;MUERHOFF, A., SCOTT;DESAI, SURESH, M.;MUSHAHWAR, ISA, K. |
分类号 |
G01N33/53;C07H21/02;C07H21/04;C12M1/34;C12N15/09;C12P19/34;C12Q1/68;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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