发明名称 |
Method for preparing modified polypeptides |
摘要 |
Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed. The methods involve a) expressing a diversified population of nucleotide sequences encoding a polypeptide of interest, b) screening the polypeptides expressed in step a) for function, immunogenicity and/or stability, c) selecting functional polypeptides having altered immunogenicity and/or increased stability, e.g. functional in vivo half-life as compared to the polypeptide of interest, and d) optionally subjecting the nucleotide sequence encoding the polypeptide selected in step c) to one or more repeated cycles of steps a)-c). In a further step the expressed polypeptides of step a) or c) can be conjugated to at least one non-polypeptide moiety.
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申请公布号 |
US2003165996(A1) |
申请公布日期 |
2003.09.04 |
申请号 |
US20020190414 |
申请日期 |
2002.07.03 |
申请人 |
HALKIER TORBEN;PEDERSEN ANDERS HJELHOLT;OKKELS JENS SIGURD;ANDERSEN KIM VILBOUR |
发明人 |
HALKIER TORBEN;PEDERSEN ANDERS HJELHOLT;OKKELS JENS SIGURD;ANDERSEN KIM VILBOUR |
分类号 |
C07H21/04;C07K1/107;C07K14/31;C07K14/47;C07K19/00;C12N15/10;C12Q1/68;G01N33/48;G01N33/50;G06F19/00;(IPC1-7):C12P21/02;G01N33/53;G06G7/48;G06G7/58 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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