| 摘要 |
Methods are disclosed for selecitng oligonucleotide probes with high target affinity. These methods include combining several types of thermodynamic criteria or nucleotide composition criteria for the selection of efficient oligonucleotide probes. The development of these methods was based on statistical analysis of datasets of hybridaization or antisense experiments. The methods described here demonstrated variable efficiency in practice, however any or all of them can be used depending on the task of probe design. |
