METHODS FOR DETERMINING A NUCLEOTIDE AT A SPECIFIC LOCATION WITHIN A NUCLEIC ACID MOLECULE
摘要
Novel methods for determining the existence or nonexistence of a test nucleotide on a strand of DNA are provided. The methods involve the use of a proofreading polymerase that is capable of incorporating a labeled nucleotide in a primer into an extension product if there is a match between the test nucleotide on the strand of DNA and the complementary nucleotide onthe primer, but which excises the labeled nucleotide and does not incorporate it into an extension product if there is a mismatch. The presence or absence of the test nucleotide then may be extablished bz determining whether the label is preserved or lost following the reaction. Methods involving the use of a quencher-chromophore pair are also provided.
申请公布号
WO02063049(A3)
申请公布日期
2003.09.04
申请号
WO2002US02908
申请日期
2002.02.01
申请人
GENOME THERAPEUTICS CORPORATION;SMITH, DOUGLAS, R.;THOMANN, HANS-ULRICH;CAHILL, PATRICK
发明人
SMITH, DOUGLAS, R.;THOMANN, HANS-ULRICH;CAHILL, PATRICK