Custom ligand design for biomolecular filtration and purification for bioseperation

摘要

The instant invention relates to a method of isolating a bio-active molecule of interest from a biological system using affinity separation. The affinity separation ligand for the bio-active molecule of interest is selected by directed molecular evolution to customize the properties of the interaction between the bio-active molecule of interest and the affinity separation ligand thereby facilitating isolation of the bio-active molecule of interest. The invention also relates to compositions comprising an affinity separation ligand bound to a solid support wherein the separation ligand is selected by the method of directed molecular evolution.