METHOD FOR THE RECOMBINATION OF GENETIC ELEMENTS

摘要

A method for the recombination of a gene is disclosed. The method involves the design of unpaired forward and reverse primers having homology to the 5' end of one template and to the 3 end of another template. Short primer extension periods results in a recombined template having paired 5' and 3' ends that can then be amplified. The amplified sample is devoid of any parental template.