发明名称 |
Electrochemical denaturation of double-stranded nucleic acid |
摘要 |
A process is described for denaturing native double-stranded nucleic acid material into its individual strands in an electrochemical cell. The process disclosed is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process may also employ a promoter compound such as methyl viologen to speed denaturation. The process may be used in the detection of nucleic acid by hybridizing with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction.
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申请公布号 |
US6613527(B1) |
申请公布日期 |
2003.09.02 |
申请号 |
US20020122139 |
申请日期 |
2002.04.15 |
申请人 |
AFFYMETRIX, INC. |
发明人 |
STANLEY CHRISTOPHER J. |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;G01N33/00 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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