Process for producing the tripeptides Ile-Pro-Pro and Val-Pro-Pro from milk casien with a peptidase and proteinase

摘要

A method for producing a tripeptide to obtain at least one of tripeptides Val-Pro-Pro and Ile-Pro-Pro comprises digesting material containing milk casein with a proteinase to produce an intermediate peptide. The intermediate peptide is selected from the group consisting of a peptide containing a sequence Val-Pro-Pro but containing no other Prop other than in this sequence and a peptide containing a sequence Ile-Pro-Pro but containing no Pro other than those in this sequence and mixtures thereof. The intermediate peptide is then digested with a peptidase to produce at least one of tripeptides Val-Pro-Pro and Ile-Pro-Pro. The method may further comprise elevating the concentration of the intermediate peptide using a hydrophobic resin after digesting the material containing the milk casein with the proteinase and before performing digestion with the peptidase. The peptidase is preferably derived from the lactic acid bacteria Lactobacillus helveticus and the proteinase is preferably selected from papain, protease A, protease M or protease P