METHOD FOR MEASURING ACTIVITY OF HEPATITIS C VIRUS REPLICASE

摘要

PURPOSE: A method for measuring activity of hepatitis C virus replicase is provided, thereby automatically carrying out HTS(High-throughput screening) analysis using SPA(Scintillation proximity assay)-based screening technologies. CONSTITUTION: A method for measuring activity of hepatitis C virus replicase comprises the steps of: mixing substrates containing isotope-labeled substrate, HCV(hepatitis C virus) replicase, SPA beads, biotin-15-UTP as a primer, poly A-500 as a template RNA, and buffer solution; carrying out the polymerization using the mixture; and measuring activity of HCV replicase using a liquid scintillation counter, wherein the isotope labeled substrate comprises 5,6-3H-UTP and another UTP; the SPA bead is streptavidin-SPA bead; and inhibitors of the polymerization process contain dimethylsulfoxide.