Strategically modified hepatitis b core proteins and their derivatives

摘要

An expression vector for the secretive production of human interferon alpha (hIFNá) comprising a polynucleotide encoding a modified E. coli thermostable enterotoxin II signal sequence and a polynucleotide encoding hIFNá) ligated to the 3'-end thereof; a microorganism transformed with the expression vector; and a process for secretively producing human interferon by culturing the microorganism, said process being capable of secreting a soluble form of active hIFNá), which does not contain an additional methionine residue at its N-terminal, into the periplasm of an E. coli cell.