发明名称 |
Method for generating highly diverse libraries |
摘要 |
A method for generating a nucleic acid library, the method involving: (a) providing a population of single-stranded nucleic acid templates, each of the templates including a coding sequence and an operably linked promoter sequence; (b) hybridizing to the population of single-stranded nucleic acid templates a mixture of substantially complementary single-stranded nucleic acid fragments, the fragments being shorter in length than the nucleic acid template; (c) contacting each of the hybridization products of step (b) with both a DNA polymerase which lacks strand displacement activity and a DNA ligase under conditions in which the fragments act as primers for the completion of a second nucleic acid strand which is substantially complementary to the nucleic acid template; and (d) contacting the products of step (c) with RNA polymerase to generate an RNA library, the library being transcribed from the second nucleic acid strand.
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申请公布号 |
NZ508287(A) |
申请公布日期 |
2003.08.29 |
申请号 |
NZ19990508287 |
申请日期 |
1999.06.29 |
申请人 |
PHYLOS, INC |
发明人 |
WAGNER, RICHARD;WRIGHT, MARTIN C;KREIDER, BRENT |
分类号 |
C12N15/09;C12N15/10;C12P19/34;C12Q1/68;(IPC1-7):C12P21/06;C12N15/74 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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