| 摘要 |
<p>Cat C3d polypeptide, fragments thereof, and nucleic acid sequences encoding the polypeptide and fragments are described. Genetic constructs encoding such polypeptides can be used to enhance the immunogenicity of antigens in cats. Variant nucleic acid sequences encoding cat C3d polypeptides, when used to express tandem arrays of the polypeptide show enhanced stability, leading to high level expression in eukaryotic and prokaryotic cell expression systems. When incorporated into a DNA immunization vector or a recombinant live organism vaccine, the variant sequences have reduced risk of undergoing homologous recombination with genomic DNA compared to wild-type sequences, thus reducing the risk of potentially damaging integration events.</p> |
