| 摘要 |
The present invention relates to improved alternatives for hot-start PCR hybridization and amplification methods by using, in an embodiment, a heat-reversible, covalent modification of nucleic acids to disrupt hybridization of primer to template, or to interfere with the ability for the polymerase enzyme to recognize nucleoside triphosphates. In an illustrative embodiment, the amino groups of guanosine have been reversibly modified by reaction with glyoxal under mild conditions.
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