| 摘要 |
<p>The present invention relates to a method which can be used for detecting mutations, in particular single nucleotide polymorphisms in parallel in different nucleotide sequences. The method comprises the procedural steps of hybridizing d(GMeATC) containing single-stranded sample nucleotide sequences with single 10 stranded reference nucleotide sequences, fixing single-stranded reference nucleotide sequences or single-stranded sample nucleotide sequences before or during the hybridization, or heteroduplexes consisting of reference and sample nucleotide sequences after or during the hybridization, on a surface, incubating with mutS, mutL and mutH for the incision of the complementary region of the is d(GMeATC)-sequence in the reference nucleic acid sequence, if a mismatch is present in the hybridized reference and sample nucleic acid sequences, removing the 3`-ends of the incised reference nucleic acids and incubating with a DNA ploymerase and with labeled esoxynucleotidetriphosphates.</p> |
