DEVICE FOR ISOELECTRIC FOCUSSING

摘要

A device for separating one or more components in a sample is disclosed, the device including: a a first planar member 1 having a channel 2 along which a sample may be loaded and components thereof focussed isoelectrically and b means for exposing the channel 2 along at least a portion of its length and thereby exposing the sample or components therewithin. The means for exposing the channel 2 may include a cover plate 7. A method of analysing a molecule is also provided, the method including: a providing an elongate open channel 2 b introducing a plurality of molecules into the elongate open channel 2 c separating molecules along the elongate open channel 2 according to their isoelectric points d accessing a molecule in the elongate open channel 2 and analysing it. A use of the device in combination with a mass spectrometer, preferably a MALDI-TOF unit, for analysis of a protein is also disclosed.