CELLULOLYTIC ENZYME GENE AND USE THEREOF

摘要

A method of treating woodchips which comprises: the step of preparing a DNA encoding an antisense RNA which is substantially complementary to the whole transcription product of a basidiomycete-origin cellulose digesting enzyme gene or a part thereof; the step of constructing a vector which contains (a) the above DNA, or (b) a recombinant DNA containing the above DNA and a DNA fragment having a promoter activity wherein the DNA is bonded to the DNA fragment in such a manner that the antisense RNA of the cellulose digesting enzyme is formed by the transcription of the DNA; the step of effecting transcription with the use of the vector and thus preparing host cells having a suppressed cellulose digesting enzyme activity; and inoculating the woodchips with the host cells having a suppressed cellulose digesting enzyme activity to thereby treat the woodchips.