| 摘要 |
The invention relates to a method for detecting mycoplasma in biological material, comprising the following steps: a) the mycoplasma are lysed in the biological material by heat treatment, b) the released mycoplasma DNA is introduced into a PCR, which uses specific primers that however recognise a segment, common to the representatives of the Mollicutes class, of the gene that codes for the 16S rRNA of mycoplasma and either c) a conventional, non-real time PCR is carried out or the reaction feed material is processed in a device for the real time PCR. The invention also relates to the control plasmid, containing primers that recognise a segment of the gene that codes for the 16S rRNA of the mycoplasma genome, said segment being specific to the species of the micro-organism class Mollicutes, but common to the individual species of the Mycoplasma genus, in addition to the majority of representatives of the genera Ureaplasma, Spiroplasma, and Acholeplasma and to an amplicon, which is located between the primers and comprises the oligonucleotide sequence 5' -cgccctactggccacctgtccaga. |
