发明名称 |
Method for the stable inversion of DNA sequence by site-specific recombination and DNA vectors and transgenic cells thereof |
摘要 |
The invention relates to a method for the stable inversion of a DNA fragment upon recombinase-mediated rearrangements using two sets of two incompatible site-specific recombinase targeting sites (SSRTS) in the same order but in reverse orientation flanking said DNA fragment to be inverted. The invention also relates to a method for the stable inversion of said DNA fragment upon rearrangement mediated by a recombinase such as Cre recombinase. The invention also relates to a method for obtaining a transgenic cell of which at least one allele of a DNA sequence of interest is invalidated by a process of conditional deletion and the genome of which comprises a reporter gene inserted at the place of the DNA fragment deleted by said process of conditional deletion. The invention also concerns a method to generate targeting sites to perform site-specific recombination mediated cassette exchange. The corresponding vectors, host cells, and transgenic animals are claimed.
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申请公布号 |
US2003159160(A1) |
申请公布日期 |
2003.08.21 |
申请号 |
US20010843150 |
申请日期 |
2001.04.27 |
申请人 |
CHAMBON PIERRE;GHYSELINCK NORBERT B.;SCHNUTGEN FRANK |
发明人 |
CHAMBON PIERRE;GHYSELINCK NORBERT B.;SCHNUTGEN FRANK |
分类号 |
C12N1/19;C12N1/21;C12N15/63;C12N15/90;(IPC1-7):A01K67/00;C12N5/06;C12N15/74 |
主分类号 |
C12N1/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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