| 摘要 |
Linear double-stranded DNA fragments containing a promoter, a nucleotide sequence, such as a transgene, preferably non-viral, and a 3' untranslated region, are delivered to tissue of an animal by direct injection accompanied by electroporation. Long-term expression of the transgene results in prolonged availability of proteins, hormones, or enzymes that may be deficient in the mammal. In addition, the linear fragments increase the safety of the vectors for mammalian gene therapy by avoiding deleterious side effects.
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