肿瘤坏死因子相关细胞凋亡诱导配体胞外区突变多肽及其制法与用途

摘要

本发明提供一种人TRAIL胞外区突变的95～281重组可溶性多肽(rsTRAIL_{Thr95Gly－281})，其第95位氨基酸从天然的苏氨酸(Thr)突变为甘氨酸(Gly)；还提供该重组可溶性多肽的制备方法以及它的抗肿瘤的用途。

主权项

1一种多肽，包括：Gly Ser Glu Glu Thr Ile Ser Thr Val Gln Glu Lys Gln Gln Asn1 5 10 15Ile Ser Pro Leu Val Arg Glu Arg Gly Pro Gln Arg Val Ala Ala16 20 25 30His Ile Thr Gly Thr Arg Gly Arg Ser Asn Thr Leu Ser Ser Pro31 35 40 45Asn Ser Lys Asn Glu Lys Ala Leu Gly Arg Lys Ile Asn Ser Trp46 50 55 60Glu Ser Ser Arg Ser Gly His Ser Phe Leu Ser Asn Leu His Leu61 65 70 75Arg Asn Glu Gly Leu Val Ile His Glu Lys Gly Phe Tyr Tyr Ile76 80 85 90Tyr Ser Gln Thr Tyr Phe Arg Phe Gln Glu Glu Ile Lys Glu Asn91 95 100 105Thr Lys Asn Asp Lys Gln Met Val Gln Tyr Ile Tyr Lys Tyr Thr106 110 115 120Ser Tyr Pro Asp Pro Ile Leu Leu Met Lys Ser Ala Arg Asn Ser121 125 130 135Cys Trp Ser Lys Asp Ala Glu Tyr Gly Leu Tyr Ser Ile Tyr Gln136 140 145 150Gly Gly Ile Phe Glu Leu Lys Glu Asn Asp Arg Ile Phe Val Ser151 155 160 165Val Thr Asn Glu His Leu Ile Asp Met Asp His Glu Ala Ser Phe166 170 175 180Phe Gly Ala Phe Leu Val Gly181 185 187