摘要 |
A method of precision calibration of magnification of scanning microscopes with the use of a test diffraction grating includes positioning and orientation of a test object on a stage of microscope so that strips of a test diffraction grating are perpendicular to a direction along which a calibration is performed, scanning of a selected portion of the test object along axes X and Y, measuring values of a signal S versus on coordinates x and y in a plane of scanning and storing of the values S (x, y) in a digital form as a two-dimensional digital array, transforming the two-dimensional array of signals S(x, y) into a two-dimensional array S (u, v) by turning of the axes so that a direction of a new axis u is perpendicular to the strips of the grating and a direction of a new axis v coincides with the strips of the grating, line-by-line mathematical processing of the array S(u) including for each line approximating of an array of discrete values S(u, v) with a periodical analytical function determining a pitch of an analytical function, and calculating a magnification in accordance with the selected direction.
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