alpha-SUBSTITUTED-alpha,beta-UNSATURATED CARBONYL COMPOUND REDUCTASE GENE

摘要

A reductase gene of an alpha-substituted-alpha,beta -unsaturated carbonyl compound which contains a DNA sequence encoding the amino acid sequence represented by SEQ ID NO:20 and the amino acid sequence represented by SEQ ID NO:21; an enzyme which is the product of the gene; a plasmid and a transformant containing the gene DNA; and a method of reducing an alpha-substituted-alpha,beta-unsaturated carbonyl compound using the transformant. Thus, it is intended to provide an enzyme gene which is useful in hydrogenating the carbon-carbon double bond in an alpha-substituted carbonyl compound having an alpha,beta-carbon-carbon double bond, which is a compound prochiral at the alpha-position, to give the corresponding alpha-substituted-alpha,beta-saturated carbonyl compound optically active at the alpha-position, and an enzyme which is the gene product.