| 摘要 |
A method for the creation of stem cells substantially equivalent to the embryonic stem cells or embryonic germ cells, but without legal or ethical complications. The method is illustrated in the context of the creation of a totipoent cell equivalent to a human fertilized egg by total asexual process. The present method uses somatic cell nuclear transfer (SCNT) technology by which the nucleus of an egg from a mammalian, other than human, was removed with care to avoid significant disruption of the egg plasma. Then a human somatic cell (any body cell other than germ cells) is arranged side by side with the de-nucleated egg. The de-nucleated egg and the human somatic cell are fused by chemical induced cell fusion, and mitochondria are transferred from the nucleus donor to the de-nucleated animal egg. After the fusion, the newly formed chimeric cell may be properly stimulated to start development, or stored cryogenically for later use. The method of the present invention will generate stem cells equivalent to the human embryonic stem cell or human embryonic germ cell without the controversy associated with the destruction of human embryos or the concern with human cloning.
|
