摘要 |
<p>A reductase gene of an α-substituted-α,β -unsaturated carbonyl compound which contains a DNA sequence encoding the amino acid sequence represented by SEQ ID NO:20 and the amino acid sequence represented by SEQ ID NO:21; an enzyme which is the product of the gene; a plasmid and a transformant containing the gene DNA; and a method of reducing an α-substituted-α,β-unsaturated carbonyl compound using the transformant. Thus, it is intended to provide an enzyme gene which is useful in hydrogenating the carbon-carbon double bond in an α-substituted carbonyl compound having an α,β-carbon-carbon double bond, which is a compound prochiral at the α-position, to give the corresponding α-substituted-α,β-saturated carbonyl compound optically active at the α-position, and an enzyme which is the gene product.</p> |