METHOD FOR DISTINGUISHING BETWEEN PROTEIN VARIANTS

摘要

Disclosed is a method for distinguishing between protein variants using high field asymmetric waveform ion mobility spectrometry (FAIMS). A method according to the instant invention includes the steps of providing a FAIMS analyzer region defined by a space between two spaced-apart electrodes, and providing predetermined conditions within said FAIMS analyzer region. Ions of an analyte protein are produced from a solution having predetermined properties and including the analyte protein, using a suitable ionization source. The ions are introduced into the FAIMS analyzer region, transmitted therethrough, and detected. An intensity of the detected ions is measured at a predetermined compensation voltage value (CV). At least a difference is determined, between the detected intensity and a reference value, the reference value relating to an expected intensity at the predetermined CV value for a reference form of the analyte protein. The analyte protein is registered as a variant of the reference form, in dependence upon the determined differences.