发明名称 |
ELECTROCHEMICAL SENSOR USING INTERCALATIVE, REDOX-ACTIVE MOIETIES |
摘要 |
Compositions and methods for electrochemical detection and localization of genetic point mutations, common DNA lesions and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential. |
申请公布号 |
WO03023365(A3) |
申请公布日期 |
2003.08.14 |
申请号 |
WO2002US29047 |
申请日期 |
2002.09.13 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
BARTON, JACQUELINE, K.;BOON, ELIZABETH, M.;KELLEY, SHANA, O.;HILL, MICHAEL, G. |
分类号 |
G01N33/53;C12N15/09;C12N15/11;C12Q1/68;G01N;G01N27/327;G01N27/416 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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