发明名称 |
Method for the detection of the antibiotic resistance spectrum of Mycobacterium species |
摘要 |
Method for the detection of the antibiotic resistance spectrum of Mycobacterium species present in a sample, possibly coupled to the identification of the Micobacterium species involved, comprising the steps of: (i) if need be releasing, isolating or concentrating the polynucleic acids present in the sample; (ii) if need be amplifying the relevant part of the antibiotic resistance genes present in said sample with at least one suitable primer pair; (iii) hybridizing the polynucleic acids of step (i) or (ii) with at least one of the rpoB gene probes, as specified in table 2, under the appropriate hybridization and wash conditions; (iv) detecting the hybrids formed in step (iii); (v) inferring the Mycobacterium antibiotic resistance spectrum, and possibly the Mycobacterium species involved from the differential hybridization signal(s) obtained in step (iv).
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申请公布号 |
US2003152982(A1) |
申请公布日期 |
2003.08.14 |
申请号 |
US20030339604 |
申请日期 |
2003.01.10 |
申请人 |
DE BEENHOUWER HANS;PORTAELS FRANCOISE;MACHTELINCKX LIEVE;JANNES GEERT;ROSSAU RUDI |
发明人 |
DE BEENHOUWER HANS;PORTAELS FRANCOISE;MACHTELINCKX LIEVE;JANNES GEERT;ROSSAU RUDI |
分类号 |
C12N15/09;C07H21/04;C12N15/00;C12Q1/68;C12R1/32;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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