发明名称 |
Method and kit for detecting intrachromosome imbalance in interphase nuclei and their applications |
摘要 |
The invention concerns a method for detecting intrachromosome imbalance in interphase nuclei, characterized in that it consists in: (a) hybridizing in situ the chromosome suspected to be affected by said imbalance using two probes marked with different fluorochromes and specific of said chromosome long arm and short arm respectively; (b) measuring the intensity of the fluorescence emitted in said nuclei with different emission wavelengths corresponding to each of the two fluorochromes; (c) calculating the ratio (Ra) between the two measured values of fluorescence intensity; and (d) comparing the resulting ratio value with at least one reference value. The invention also concerns a kit for implementing said method and uses of said kit and said method, in particular for detecting cancer cells in a biological sample and for diagnosing cancer pathologies.
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申请公布号 |
US6605436(B1) |
申请公布日期 |
2003.08.12 |
申请号 |
US20010805768 |
申请日期 |
2001.07.06 |
申请人 |
COMMISSARIAT A L'ENERGIE ATOMIQUE;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;INSTITUT CURIE |
发明人 |
TRUONG KHUONG;MALFOY BERNARD;BOURGEOIS CLAIRE;VIELH PHILIPPE;GUILLY MARIE-NOELLE;DUTRILLAUX BERNARD |
分类号 |
G01N33/566;C12N15/09;C12Q1/68;G01N21/78;G01N33/58;(IPC1-7):C12Q1/68;G01N33/574;C12P19/34;C07H21/02;C07H21/04 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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