发明名称 |
Methods for isolating one strand of a double-stranded nucleic acid |
摘要 |
The present invention provides methods and kits for isolating one strand of a double-stranded target nucleic acid. The method capitalizes on the differences in the kinetics and thermodynamic stabilities between conventional DNA/DNA, DNA/RNA and RNA/RNA duplexes and heteroduplexes in which one strand of the heteroduplexe is a nucleobase polymer having a net positively charged or net neutral backbone, such as a PNA.
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申请公布号 |
US2003148277(A1) |
申请公布日期 |
2003.08.07 |
申请号 |
US20010909001 |
申请日期 |
2001.07.18 |
申请人 |
CHIESA CLAUDIA;SCHROTH GARY P.;EGHOLM MICHAEL |
发明人 |
CHIESA CLAUDIA;SCHROTH GARY P.;EGHOLM MICHAEL |
分类号 |
C12N15/09;C12N15/10;(IPC1-7):C12Q1/68;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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