| 摘要 |
<p>In a single reaction, thousands of different nucleic acid sequences can be detected and quantified, with a sensitivity to as few as 10 target copies per sample, by a method that incorporates specific hybridization and rolling circle amplification. This approach offers greater accuracy because the amount of reaction product generated at the end of amplification is strictly proportional to and dependent on the amount of target nucleic acid, and sample-to-sample variability is eliminated through the use of a universal template and primer. The approach can be adapted to the detection of point mutations as such and to identifying the presence of genetic mutations for purposes of research and diagnosis, respectively.</p> |
