The invention relates to methods and reagents for identifying/isolating protein targets of chemical compounds (for example, drug candidates) using mass spectrometry. The invention provides a method for capturing and identifying proteins using tethered small-molecule probes. This technology also allows the market expansion of known drugs by finding new therapeutic targets; identification of the mechanism of toxicity of drug candidates or drugs which failed in the clinic; identification of new chemical tools for chemically-driven target validation; identification of new drug leads; and identification of the mechanism of action of drugs and drug candidates. A ke y advantage of the technology is that a single experiment can identify the numerous proteins which interact with a probe (or "bait").
申请公布号
CA2474469(A1)
申请公布日期
2003.08.07
申请号
CA20032474469
申请日期
2003.01.28
申请人
MDS PROTEOMICS, INC.
发明人
TOLEDO-SHERMAN, LETICIA M.;SUCHOLEIKI, IRVING;CLIMIE, SHANE;HOSFIELD, CHRISTOPHER MARK;DUEWEL, HENRY S.;DESOUZA, LEROI VICTOR;LIAO, LINDA CHIOU-LIN;MCBROOM, LINDA D. B.