METHOD FOR EXPRESSION OF PROTEINS ON SPORE SURFACE

摘要

PURPOSE: A method for expression of proteins on spore surface is provided, thereby effectively solving problems of the prior methods, and consequently producing the spore surface protein expression system having improved stability to physicochemical changes. CONSTITUTION: A method for expression of proteins on spore surface comprises the steps of: (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding a protein of interest, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the protein of interest, (ii) transforming a host cell with the vector for spore surface display; (iii) displaying the protein of interest on a surface of a spore of the host cell; and (iv) recovering the spore displaying on its surface of the protein of interest.