SCREENING METHODS BASED ON TRANSCRIPTIONAL REGULATION ANALYSIS SYSTEM USING TRANSFORMED CELL LINES

摘要

PURPOSE: Screening methods based on transcriptional regulation analysis system using transformed cell lines are provided, thereby rapidly screening substances affecting the transcription activity. CONSTITUTION: A method for screening substances affecting transcription activity associated with a promoter comprises the steps of: (a) treating a medium containing eukaryotic cells transfected stably with a reporter vector with a sample to be assayed, in which the reporter vector carries an eukaryotic replication origin, a promoter and a reporter gene being located downstream of the promoter; and (b) measuring an activity or a quantity of protein encoded by the reporter gene on the reporter vector. A method for screening substances affecting transcription activity associated with an enhancer comprises the steps of: (a) treating a medium containing eukaryotic cells transfected stably with a reporter vector with a sample to be assayed, in which the reporter vector carries an eukaryotic replication origin, a promoter, an enhancer being located upstream of the promoter and a reporter gene being located downstream of the promoter; and (b) measuring an activity or a quantity of protein encoded by the reporter gene on the reporter vector.