Method of reducing baseline instabilities in liquid chromatographic measurements and liquid chromatography apparatus

摘要

A method of reducing baseline instabilities in liquid chromatographic measurements is disclosed. A mobile phase comprising sample substances passes through a chromatographic column comprising a stationary phase, and subsequently through a detector. An additional solid body or liquid bath having a high heat capacity and heat conductance is coupled to the column and to an inlet capillary of the column such that the inlet capillary and the column are enclosed by said solid body or liquid bath. In that way temperature stabilization of the column and inlet capillaries is achieved and baseline variations due to temperature dependent adsorption of water at the stationary phase, in particular stationary phases with amino functional groups, are reduced.