发明名称 |
HUMAN PAPILLOMAVIRUS E2 TRANSACTIVATION DOMAIN/INHIBITOR CO-CRYSTAL AND X-RAY COORDINATES DEFINING THE INHIBITOR-BINDING POCKET |
摘要 |
A crystallizable composition, comprising an PV E2 TAD-like polypeptide of SEQ ID NO.2 complexed with an inhibitor L. The invention also provides a method for producing the crystallized HPV E2 TAD-inhibitor complex (HPV E2 TAD-L) comprising: a) mixing purified HPV E2 TAD, contained in a purification buffer, with solublized inhibitor L to generate a complex solution containing the HPV E2 TAD-L complex; and b) crystallizing the complex from a) in a crystallization buffer. The invention also provides a method for producing crystallized apo HPV E2 TAD, comprising: a) mixing apo HPV E2 TAD, contained in a purification buffer, with a crystallization buffer.X-ray crystal structure coordinates the HPV E2 TAD-L complex, are also provided, which define an inhibitor binding pocket. The inhibitor binding pocket is useful for screening potential small molecule inhibitors that bind to the pocket. |
申请公布号 |
WO03006495(A3) |
申请公布日期 |
2003.07.31 |
申请号 |
WO2002CA01058 |
申请日期 |
2002.07.12 |
申请人 |
BOEHRINGER INGELHEIM INTERNATIONAL GMBH;CAMERON, DALE, R.;ARCHAMBAULT, JACQUES;YOAKIM, CHRISTIANE;WHITE, PETER;WANG, YONG |
发明人 |
CAMERON, DALE, R.;ARCHAMBAULT, JACQUES;YOAKIM, CHRISTIANE;WHITE, PETER |
分类号 |
C12N15/09;A61K45/00;A61P15/00;A61P17/12;A61P31/20;A61P35/00;C07D405/12;C07D417/12;C07K14/025 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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