发明名称 |
DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS USING PLANAR WAVEGUIDES |
摘要 |
The present invention relates to a method of detecting single nucleotide polymorphisms in a gene of interest. A plurality of probes is immobilized on a planar waveguide. The probes comprise sequences complementary to a wildtype sequence of the gene of interest and complementary to a sequence of a known SNP in the gene of interest. A fluorescently-labeled analyte is flowed over the planar waveguide. The binding between the labeled analyte and each of the probes causes a change in the fluorescence signal. The SNP is detected by comparing the hybridization kinetics of the analyte with each of the probes. A method of detecting single nucleotide polymorphisms in a gene of interest by sequencing by hybridization is also disclosed.
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申请公布号 |
CA2473558(A1) |
申请公布日期 |
2003.07.31 |
申请号 |
CA20032473558 |
申请日期 |
2003.01.17 |
申请人 |
UNIVERSITY OF UTAH RESEARCH FOUNDATION |
发明人 |
WANG, HSU-KUN;HERRON, JAMES N.;TOLLEY, SAMUEL E. |
分类号 |
G01N33/50;C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/50 |
代理机构 |
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地址 |
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