PRIMERS FOR AMPLIFYING GENE HSP65 OF MYCOBACTERIUM SPECIES AND IDENTIFICATION METHOD OF MYCOBACTERIUM SPECIES USING THE SAME

摘要

PURPOSE: Primers for amplifying gene hsp65 of Mycobacterium species and an identification method of Mycobacterium species using the same are provided, thereby rapidly and easily detecting and identifying Mycobacterium species. CONSTITUTION: A pair of primers for specifically amplifying gene hsp65 of Mycobacterium species have the nucleotide sequences of SEQ ID NOs: 1 and 2. A method for identifying Mycobacterium species comprises amplifying the gene hsp65 fragments of Mycobacterium species using the primers of SEQ ID NOs: 1 and 2; and subjecting the amplified gene fragments to RPLP(restriction fragment length polymorphism) analysis using the specific restriction enzyme recognition site within the amplified fragments, wherein the restriction enzyme is Xho I.