PRODUCTION OF RECOMBINANT THERAPEUTIC BIOSCAVENGERS AGAINST CHEMICAL AND BIOLOGICAL AGENTS

摘要

This application provides a method to produce recombinant bioscavenger molecules to be used as human treatments to protect against toxicity resulting from exposure to chemical/biological agent toxins or drugs. This invention relates to the production of glycoproteins that exhibit poor stability in vivo and are thus inadequate as therapeutic treatments without the additional post-translational modifications of the expressed molecules. In one embodiment, the method combines molecular and biochemical technologies; first for the expression of recombinant molecules and second for the in vitro glycoslyation of the purified or partially purified expressed molecules, intended to mimic the glycoprotein profiles of the native molecules. In another embodiment, post-translation modifications can be provided by direct genetic modification of the cells used in the protein expression system. Although the invention is intended for in vivo use, the invention allows for decontamination in vitro. The establishment of recombinant detoxification agents has applications in numerous terrorist, drug, and environmental scenarios involving military and civilian welfare.