发明名称 |
Transfection of eukaryontic cells with linear polynucleotides by electroporation |
摘要 |
The present invention provides an improved method for gene delivery in eukaryotic cells by electroporation, preferably in human hematopoietic cells, particular dendritic cells. The method of the invention is superior to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for gene delivery, including tumor antigen loading of dendritic cells.
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申请公布号 |
US2003143743(A1) |
申请公布日期 |
2003.07.31 |
申请号 |
US20020177390 |
申请日期 |
2002.06.20 |
申请人 |
SCHULER GEROLD;BERNEMAN ZWI N.;VAN TENDELOO VIGGO F. I.;PONSAERTS PETER;STROBEL ISOLDE |
发明人 |
SCHULER GEROLD;BERNEMAN ZWI N.;VAN TENDELOO VIGGO F. I.;PONSAERTS PETER;STROBEL ISOLDE |
分类号 |
C12N15/87;(IPC1-7):C12N15/87 |
主分类号 |
C12N15/87 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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