发明名称 |
PROBE CORRECTION FOR GENE EXPRESSION LEVEL DETECTION |
摘要 |
Individual probes on micro-arrays are re-scaled and corrected with a set of probe dependent coefficients derived from genomic-DNA hybridization signals. A dynamic range for gDNA binding is determined by measuring a concentration signal curve. Signals for each probe are measured during multiple hybridizations within a linear range. Concentration insensitive probes are then found for two sets of experiments. Probes are discarded based on a threshold compared to their standard deviation divided by their average in each set. The correction coefficients are used to calculate a corrected intensity for each probe. Probes having high uncertainty (0.5 in one embodiment) are discarded. A weighting factor for each probe is determined along with an uncertainty factor. Finally, a call for each gene is made, such as absent, marginal or present.
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申请公布号 |
CA2472733(A1) |
申请公布日期 |
2003.07.31 |
申请号 |
CA20032472733 |
申请日期 |
2003.01.17 |
申请人 |
SYNGENTA PARTICIPATIONS AG |
发明人 |
WANG, XUN;FAN, YIPING;CHANG, HUR-SONG;ZOU, GUANGZHOU;ZHU, TONG;LONG, FAN |
分类号 |
G01N33/566;C07H21/02;C07H21/04;C12N15/09;C12P19/34;C12Q;C12Q1/68;G01N31/00;G01N33/48;G01N33/50;G01N33/53;G06F15/00;G06F17/00;G06F19/20;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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