发明名称 |
METHODS AND COMPOSITIONS FOR DETECTING CANCERS ASSOCIATED WITH METHYLATION OF hMLH1PROMOTER DNA |
摘要 |
Methods are provided for detection of cancers associated with methylation of hMLH1 promoter DNA in a subject. The method comprise assaying for the presence of methylated hMLH1 promoter DNA in a bodily fluid from a subject. In one embodiment, the method comprises reacting DNA from the sample with a chemical compound that converts non-methylated cytosine bases, to a different nucleotide base. The compound-converted DNA is then amplified using a methylation-sensitive polymerase chain reaction (MSP) employing primers that amplify the compound-converted DNA template. The present invention also provides nucleotide primer sequences for use in the methylation-sensitive PCR assay. |
申请公布号 |
WO0224056(A3) |
申请公布日期 |
2003.07.31 |
申请号 |
WO2001US29713 |
申请日期 |
2001.09.20 |
申请人 |
CASE WESTERN RESERVE UNIVERSITY;MARKOWITZ, SANFORD;GRADY, WILLIAM |
发明人 |
MARKOWITZ, SANFORD;GRADY, WILLIAM |
分类号 |
A61B;C07H21/02;C07H21/04;C12P19/34;C12Q1/68 |
主分类号 |
A61B |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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