Recombinant plant expression vector comprising isolated cDNA nucleotide sequence encoding farnesyl pyrophosphate synthase (FPS) derived from seedlings of sunflower (Helianthus annus)

摘要

The present invention provides nucleotide and peptide sequences of an isolated cDNA coding for sunflower farnesyl pyrophosphate synthase, a key enzyme for the structurally diverse class of isoprenoid biosynthetic metabolites. The present invention also provides the recombinant plant expression vector comprising said nucleotide sequences and for the host cell into which said DNA sequence in the recombinant plant expression vector has been introduced to produce transgenic tobacco plants. Transgenic plants expressing heterologous farnesyl pyrophosphate synthase show 2-fold increase in seed production, higher chlorophyll contents in leaves as compared to non-transgenic or control vector-transformed tobacco plants. The expression vector for the sunflower farnesyl pyrophosphate synthase gene, herein designated pCIP-sfps, has been deposited with the Korea Research Institute of Bioscience and Biotechnology Korean Collection for Type Cultures (K.C.T.C.) designation number KCTC 0520BP, having been deposited on Sep. 7, 1998.