| 摘要 |
The present invention is directed to a method of detecting over-expression of certainT-cell receptor V genes in a sample. The method uses a T-cell receptor gene arraycontaining a substrate with a plurality of positions, each position having an immobilizednucleic acid complementary to a fragment of various families of the human T-cellreceptor V genes. Nucleic acids are extracted from a sample and labelled, then contactedwith the T-cell receptor gene array to allow complementary sequences to hybridize.After the unhybridized nucleic acids are removed, the one or more positions that haveelevated signals are identified and the over-expressed T-cell receptor V genes aredetected. The present invention is also directed to a kit comprising the T-cell receptorgene array for detecting over-expression of certain T-cell receptor V genes in a sample.The present invention is useful in diagnosing autoimmune diseases such as multiplesclerosis, rheumatoid arthritis, insulin-dependent diabetes mellitus, type I diabetes,inflammatory bowel disease, psoriasis, system lupus erythematosus, and Crohn'sdisease or T cell associated malignancies such as T cell leukemia and T cell lymphoma. |
