发明名称 |
System and method for temperature gradient capillary electrophoresis |
摘要 |
The present invention relates to a method for determining the presence of a mutation in a first sample comprising first nucleotides. The reference sample comprising reference nucleotides. The first sample and a reference sample are subjected to electrophoresis in the presence of at least one intercalating dye. During electrophoresis the temperature of the first sample and the reference sample is changed by an amount sufficient to change an electrophoretic mobility of at least one of the first or reference nucleotides. Fluorescence intensity data are obtained. The fluorescence intensity data are indicative of the presence of the first and reference nucleotides. At least one of the first sample or reference samples comprises products resulting from a polymerase chain reaction (PCR), the products not having been desalted prior to electrophoresis.
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申请公布号 |
US2003138821(A1) |
申请公布日期 |
2003.07.24 |
申请号 |
US20020287826 |
申请日期 |
2002.11.05 |
申请人 |
GUO ZHIYONG;LIU ZHAOWEI;LI QINGBO |
发明人 |
GUO ZHIYONG;LIU ZHAOWEI;LI QINGBO |
分类号 |
C12Q1/68;G01N27/447;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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