ARRAYED POLYNUCLEOTIDES AND THEIR USE IN GENOME ANALYSIS

摘要

A method for determining a single nucleotide polymorphism present in a genome, comprises the steps of: (i) immobilising fragments of said genome onto the surface of a solid support to form an array of polynucleotide molecules capable of interrogation, wherein the array allows the molecules to be individually resolved by optical microscopy, and wherein each molecule is immobilised by covalent bonding to the surface, other than at part of each molecule that can be interrogated; (ii) identifying nucleotides at selected positions in the genome; and (iii) comparing the results of step (ii) with a known consensus sequence, and identifying any differences between the consensus sequence and said genome.