SECRETORY SIGNAL SEQUENCE ST II (VARIANTS), NUCLEIC ACID ENCODING ITS, PLASMID VECTOR, METHOD FOR OPTIMIZATION OF SECRETION AND METHOD FOR SECRETION OF HETEROLOGOUS POLYPEPTIDE

摘要

FIELD: genetic engineering, molecular biology. SUBSTANCE: invention represents variants of nucleic acids comprising secretory signal sequence ST II. Nucleotide sequences are given in the description. Also, invention compares levels of expression of polypeptides regulating by set of variants of nucleic acids encoding secretory signal sequence ST II and reveals variants providing effective translation that determines optimal secretion of matured polypeptide. Procaryotic host-cell is transformed with vector including secretory signal sequence ST II bound functionally to nucleic acid encoding the end heterologous polypeptide. Invention ensures to provide the complete conversion of precursor to the matured product and high levels of the end polypeptide in the case of reduction of translation effectiveness. EFFECT: improved method of optimization and secretion of polypeptide. 32 cl, 22 dwg, 2 tbl, 1 ex