AN ISOLATED NUCLEOTIDE SEQUENCE RESPONSIBLE FOR THE TOMATO DARK GREEN (DG) MUTATION AND USES THEREOF

摘要

The present invention describes an isolated nucleotide sequence responsible for the tomato dark green (dg) phenotype, wherein said sequence comprises an altered tomato DET1 gene sequence or fragment thereof. Further described is a method for detecting the presence of the dg mutation in a plant, comprising the steps of isolating the genomic DNA from said plant, amplifying a gene fragment containing said dg mutation from said genomic DNA by use of a PCR technique and determining the presence of said dg mutation in said genomic DNA. Further described is a method for preparing double-mutant lines of Lycopersicon esculentum having genotype dg/dg p/p, wherein p represents any recessive photomorphogenic mutation that is genetically unlinked to the dg mutation.