| 摘要 |
A flow-through assay for detecting the quantity of an analyte residing in a test sample is provided. The flow-through assay contains a porous membrane that is in fluid communication with probe conjugates that contain a specific binding member and a detectable probe. The porous membrane also defines a detection zone and a calibration zone. The calibration zone includes two or more calibration regions (e.g., lines, dots, etc.) containing differing amounts of a binder that is configured to bind with the probe conjugates. As a result, calibration signals are generated that can be readily compared (visually, quantitatively, and the like) to a detection signal to determine the presence or quantity of an analyte in the test sample.
|
